Publications

  1. Wilson, KM, Siegal, G, Lord, EM "Tumor Necrosis Factor - Production by Tumor Associated Macrophages and Tumor Sensitivity" FASEB JOURNAL, 2, 1988, p.A 695                                                                                                                  

  2. Wilson, K., Siegal, G. and Lord, E. "Tumour Necrosis Factor-Mediated Cytotoxicity by Tumour- Associated Macrophages", Cellular Immunology, 123, 1989, pp.158-165                                                                                                                    

  3. Siegal, G., Turchi, J., Mallaber, L., Jessee, B., Bambara, R., and Myers, T. "Structural Relationship Between Two Forms of DNA Polymerase Epsilon from Calf Thymus", J. Biol. Chem., 267, 1992, pp.3991-3999 complete paper (PDF format)

  4. Siegal, G., Turchi, J., Jessee, B., Myers, T., and Bambara, R. "A Novel DNA Helicase from Calf Thymus", J. Biol. Chem., 267, 1992, pp.13629-13635 complete paper (PDF format)

  5. Siegal, G., Myers, T. Turchi, J., and Bambara, R. "A 5' to 3' Exonuclease Functionally Interacts with Calf DNA Polymerase Epsilon", Proc. Natl. Acad. Sci. USA, 89, 1992, pp.9377-9381 complete paper (PDF format)

  6. Turchi, J., Siegal, G., and Bambara, R. "DNA Helicase E and DNA Polymerase Epsilon Functionally Interact for Displacement Synthesis" Biochemistry, 31, 1992, pp. 9008-9015 complete paper (PDF format)

  7. Dötsch, V., Wider, G., Siegal, G. and Wüthrich, K. "Interaction of Urea with an Unfolded Protein, the DNA-binding Domain of the 434-Repressor", FEBS Letts. 366, 1995, pp. 6-10 complete paper (PDF format)

  8. Dötsch, V., Wider, G., Siegal, G. and Wüthrich, K. "Salt-Stabilized Globular Protein Structure in 7 M Aqueous Urea Solution", FEBS Letts. 372, 1995, pp. 288-290 complete paper (PDF format)

  9. Pervushin, K., Billeter, M., Siegal, G., and Wüthrich, K. "Structural Role of a Buried Salt Bridge in the 434 Repressor DNA-Binding Domain" J. Mol. Biol., 264, 1996 pp.1002-1012

  10. Driscoll, P., C. and Siegal, G. "NMR of Natural Molecules" in Nuclear Magnetic Resonance, 26, senior reporter G. A. Webb, Royal Society of Chemistry Information Services, Cambridge, UK

  11. Siegal, G., Davis, B., Kristensen, S., Sankar, A., Panayotou, G., Linacre, J., Stein, R., Waterfield, M.,D. and Driscoll, P.C. "Solution Structure of the C-Terminal SH2 Domain of the p85a Regulatory Subunit of Phosphoinositide 3-Kinase" J. Mol. Biol., 276, 1998, pp.461-478 complete paper (PDF format)

  12. Perez, J., Kriek, J., Dijk, J., Möller,W. and Siegal, G., Hård, K., Kalverda, A., Canters, G.W., "1H, 15N and 13C chemical shift assignment of the guanine nucleotide exchange domain of human Elongation Factor-one beta" J. Biomol. NMR., 12, 1998, pp. 467-468 complete paper (PDF format)

  13. Siegal, G. "The Surprisingly Flexible PTB Domain" Nature Structural Biology, 6, 1999, pp.7-10 complete paper (PDF format)

  14. Perez, J.M.J., Siegal, G., Kriek, J., Hård, K., Dijk, J., Canters, G.W. and Möller,W., "The solution structure of the guanine nucleotide exchange domain of human elongation factor-one beta reveals a striking resemblance to that of EF-Ts from E. coli" Structure, 2, 1999, pp.217-226 complete paper (PDF format)

  15. Lo Surdo, P., Bottomley, M.J., Arcaro, A., Siegal, G., Panayotou, G., Sankar, A., Gaffney, P.R.J., Potter, B.V.L., Riley, A.M. Waterfield, M.D. and Driscoll, P.C."Structural and biochemical studies of the interactions of the p85alpha phosphoinositide 3-kinase SH2 domains with phosphoinositides and inositol polyphosphates" J. Biol. Chem. 274, 1999, pp.15678-15685 complete paper (PDF format)

  16. Kolczak, U., Salgado, J., Siegal, G., Saraste, M. and G.W. Canters "Paramagnetic NMR Studies of Blue and Purple Copper Proteins" Biospectroscopy. 5, 1999, S19-S32 complete paper (PDF format)

  17. Hoedemaeker, F.J., Siegal, G., Roe, S.M., Driscoll, P.C. and J.P.A. Abrahams "Crystal Structure of the C-Terminal SH2 Domain of the p85alpha Regulatory Subunit of Phosphoinositide 3-Kinase: An SH2 domain mimicking its own substrate" J. Mol. Biol. 292, 1999, pp.763-770 complete paper (PDF format)

  18. Siegal, G., van Duynhoven , J. and Baldus, M., "Biomolecular NMR: Recent Advances in Liquids, Solids and Screening" Curr. Opin. Chem. Biol. 3, 1999, pp.530-536 complete paper (PDF format)

  19. Kristensen, S., Siegal, G., Davis, B.J., Sankar, A., and Driscoll, P.C., "Backbone dynamics of the C-Terminal SH2 Domain of the p85alpha Subunit of Phosphoinositide-3 Kinase. Effect of Phosphotyrosine Peptide Binding and Characterization of Slow Conformational Exchange Processes" J. Mol. Biol, 299, 2000, pp. 771-788 complete paper (PDF format)

  20. B. Aguilera, G. Siegal, H.S. Overkleeft, N.J. Meewenoord, F.P.J.T. Rutjes, J. C.M. van Hest, H. E. Schoemaker, G.A. van der Marel, J. H. van Boom, M. Overhand., "Synthesis and Structural Analysis of a a b-hairpin modified Peptide containing a Sugar Amino Acid" Eur. J. Org. Chem., 8, 2001, pp. 1541-1547 complete paper (PDF format)

  21. Hilge, M., Siegal, G., Vuister, GW., Güntert, P. Gloor, S., Abrahams, JP "ATP-induced conformational changes of the nucleotide binding domain of Na,K-ATPase" Nature Structural Biology, 10, 2003, pp. complete paper (PDF format)

  22. Vanwetswinkel. S, Kriek, J., Andersen, GR. and Siegal, G "1H, 15N and 13C chemical shift assignment of the highly conserved 19 kDa C-terminal domain from human Elongation Factor 1Bg " J. Biomol. NMR., 26, 2003, pp. 189-190 complete paper (PDF format)
     

  23. van Well, R. M.; Marinelli, L.; Altona, C.; Erkelens, K.; Siegal, G.; van Raaij, M.; Llamas-Saiz, A. L.; Kessler, H.; Novellino, E.; Lavecchia, A.; van Boom, J. H.; Overhand, M. "Conformational Analysis of Furanoid epsilon-Sugar Amino Acid Containing Cyclic Peptides by NMR Spectroscopy, Molecular Dynamics Simulation, and X-ray Crystallography: Evidence for a Novel Turn Structure" J. Am. Chem. Soc., 125, 2003, pp.10822 complete paper (PDF format)
     

  24. Vanwetswinkel, S., Kriek, J., Andersen, G.R., Güntert, P., Dijk, J., Canters,G.W. and Siegal. G., "Solution structure of the 162 residue C-terminal domain of human Elongation Factor 1Bgamma", J. Biol. Chem., 278, 2003, pp.43443 complete paper (PDF format)

  25. Vanwetswinkel, S. Heetebrij, R.J., van Duynhoven, J., Hollander,  J.G., Filippov, D.V., Hajduk, P. J.  and Siegal, G., "TINS: Target Immobilized NMR Screening – An efficient and sensitive new method for ligand discovery", Chem. Biol., 12, 2005, pp.207-216 complete paper (PDF format) see the commentary in Nature Methods

  26. Kobayashi, M. and Siegal, G., "1H, 15N, and 13C resonance assignments of the BRCT region of the large subunit of human Replication Factor C", J. Biomol. NMR, 31, 2005, pp. 183-184. complete paper (PDF format)

  27. Masakazu Kobayashi, Francis Figaroa, Nico J. Meeuwenoord, Lars E. T. Jansen, and Gregg Siegal, "Characterization of the DNA binding and structural properties of the BRCT region of human replication factor C p140 subunit"
    J. Biol. Chem.,281,7,2006, pp. 4308-4317 complete paper (PDF format)

  28. Adriaan W. Tuin, Gregg Siegal, Gijsbert A. van der Marel, Herman S. Overkleeft, Dmitri V. Filippov, "Facile synthesis and application of uniformly-13C, 15N - labeled phosphotyrosine for ligand binding studies", Bioorg. Med. Chem. Lett., 16,7, 2006, pp. 3807-3808, complete paper (PDF)

  29. Thorsten Marquardsen, Martin Hofmann, Johan G. Hollander, Caroline M.P. Loch, Suzanne R. Kiihne, Frank Engelke, Gregg Siegal, "Development of a dual cell, flow-injection sample holder and NMR probe for comparative ligand-binding studies", J. Mag. Reson., 2006, 182, pp. 55-65 complete paper (PDF format)

  30. Röhrig,C.H.; Loch,C.M.P.; Guan,J.Y.; Siegal,G.; Overhand,M. "Fragment-Based Synthesis and SAR of Modified FKBP Ligands: Influence of Different Linking on Binding Affinity", ChemMedChem, 2, 7, 2007, p. 1054-1070, complete paper (PDF format)

  31. Siegal, G., AB, Eiso and Schultz, J., "Integration of Fragment Screening and Library Design", Drug Discovery Today, 2007, 12, p.1032-1039, complete paper (PDF format)

  32. Pellecchia, M. et al. and Siegal,G., "Perspectives on NMR in drug discovery: a technique comes of age", Nature Reviews Drug Discovery, 2008, 7, p.738, complete paper (PDF paper)

  33. Siegal,G. and Hollander,J.G., "Target Immobilization and NMR Screening of Fragments in Early Drug Discovery", Current Topics in Medicinal Chemistry, 2009, 9, p.1736, complete paper

  34. Kobayashi,M., AB, E., Bonvin, A.M.J.J., and Siegal, G, "Structure of the DNA bound BRCT region from human Replication Factor C p140 and model of the protein-DNA complex", J. Biol. Chem., 2010, 285, p. 10087

  35. Kobayashi,M., Retra. K., Figaroa, F., Hollander,J.G., AB, E., Heetebrij, R.J., Irth, H. and Siegal, G. "Target Immobilization as a Strategy for NMR-Based Fragment Screening: Comparison of TINS, STD, and SPR for Fragment Hit Identification", J. Biomol. Screening, in press

  36. Früh, V., Zhou,Y., Dan Chen, D., Loch,C., AB, E., Grinkova, Y.N., Verheij, H., Sligar,S.G., Bushweller,J.H. and Siegal, G. "Application of Fragment-Based Drug Discovery to Membrane Proteins: Identification of Ligands of the Integral Membrane Enzyme DsbB", Chemistry & Biology (2010), doi:10.1016/j.chembiol.2010.06.011

  37. Früh, V., Ijzerman, A.P. and Siegal, G., "How to catch a membrane protein in action: A review of functional membrane protein immobilization strategies and their applications", Chemical Reviews, in press

  38. Overmeer R.M., Gourdin, A.M., Giglia-Mari, A., Siegal, G., Fousteri, M., Mullenders, L.H.F. and Vermeulen, W., "RFC recruits DNA polymerase δ to sites of NER but is not required for PCNA recruitment", Mol. Cell Biol., in press

     

     

    39.

Book Chapters

Früh, V., Heetebrij, R.J. and Siegal, G. "Target Immobilized NMR Screening: Validation and Extension to Membrane Proteins" in Fragment-Based Drug Discovery: Practical Aspects, E.R. Zartler and M.J. Shapiro , Eds, Wiley

 

 

 

 



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